Recently, top journals in biology area 1New PhytologistPublished bet365 online sports betting titled "Tomato defense againstMeloidogyne incognitaby jasmonic acid-mediated fine-tuning of kaempferol homeostasis" research paper,Elucidated that JA finely controls the content of kaempferol in the underground parts of tomatoes by regulating the flavonol synthesis repressor MYB57 and the activator MYB108/112,To balance the development of lateral roots and the improvement of resistance。This research is led by the facility horticulture research team of our school,Professor Wang Shaohui and Associate Professor Zhao Wenchao are the corresponding authors of the paper,Liang Jingjing, a master’s degree graduate from our school (currently a PhD candidate at China Agricultural University),Associate Professor Zhao Wenchao、Associate Professor Huang Huang is the co-first author of the paper。Yang Jinshan, a graduate student of our school、Feng Jiaping、bet365 online sports betting mobileZhao Mengjia,Associate Professor Sun Lulu,Senior Experimenter Yang Rui、Wang Jianli,Also participated in some research work。This research was funded by the National Key Research and Development Program and the National Natural Science Foundation of China。
This study is the first to prove the JA synthesis geneLoxDOverexpression significantly improves tomato resistance to nematodes。Pass avoidance test,ProofLoxDThe nematode invasion rate of the gene overexpression strain is significantly lower than that of the wild type。YesLoxDMetabolome analysis of root exudates from overexpressed and wild-type tomatoes,The flavonoids in the root exudates of the overexpression lines were significantly increased。One-on-one nematode avoidance and movement inhibition experiments,Confirmed that flavonoids (kaempferol) inhibit the movement ability of nematodes,And affects the invasion rate of nematodes。At the same time,DiscoveredLoxDOverexpression promotes kaempferol synthesis pathwayFLS、F3HandCHISignificant upregulation of gene expression,And the FLS overexpression line significantly improved the resistance to root-knot nematodes。Use transcriptome to mine and prove the MYB transcription factor MYB57 that regulates kaempferol synthesis in tomato roots、MYB108 and MYB112 are bet365 bet365 online sports betting casinoall induced by external JA and root-knot nematodes。In vivo and in vitro experiments prove that MYB57 interacts with multiple JAZ proteins in the jasmonic acid signaling pathway。Reverse genetics test proof,MYB57 negatively regulates kaempferol synthesis and root-knot nematode resistance,Positively regulates root growth;MYB108 and 112 are just the opposite。This shows that kaempferol is an important secondary metabolite and signaling substance in plants,Strictly controlled。Jasmonic acid signaling regulates kaempferol levels at multiple levels simultaneously,To maintain the balance of stress resistance and growth and development in the body。
Working model of jasmonate regulating tomato resistance to root-knot nematode
at,In this study, the author also discussed the duality of JA in the interaction between plants and root-knot nematodes。Low concentrations of JA can be utilized by root-knot nematodes,Promote the formation of feeding sites,Conducive to the parasitism of root-knot nematodes。High concentration of JA prevents the invasion of root-knot nematodes,Inhibits root knot formation and nematode parasitism。