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Time: 2022-07-07 Author: Source: View:

Recently,Ornamental Plant Research published online an article titled Key microRNAs and target genes involved in regulating maturation in by the team of Professor Zhang Kezhong from the School of Forestry on our campus.Lilium’s research article。This research provides new ideas for revealing the phase change mechanism of lily,And provides important scientific basis for the genetic improvement of shortening the juvenile period of lily bulbs。Professor Zhang Kezhong from the School of Forestry on our campus and the Advanced Innovation Center for Molecular Design Breeding of Forest Trees、Associate Professor Ge Wei of the School of Landscape Architecture is the corresponding author of this article,Master Chen Yao is the first author。

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According to introduction,Lily is a famous ornamental plant,But its infancy is too long。Under natural conditions,Small balls propagated by cuttings or tissue culture,Infancy in the first year,Only one leaf grows;After 1-2 bet365 online sports betting mobileyears of growth,Entering adulthood,The main stem begins to grow,Multiple leaves growing on the main stem,Assimilation product accumulation growth rate,Bulls expand faster。Under normal conditions,It takes at least 3-4 years to cultivate small lily balls into large commercial balls,Resulting in high bulb breeding costs。Research findings by Professor Zhang Kezhong’s team,Through special temperature change treatment,Can induce phase change in lily balls,Shorten the childhood period,Entering adulthood early,However, its molecular regulatory mechanism remains to be further studied。Therefore,Studying the molecular regulatory mechanism of phase transition in lily,For shortening the juvenile period of lilies、It is of great significance to promote development and maturity and accelerate the cultivation process of commercial large balls。

This paper uses omics joint analysis to screen out the key modules that regulate the phase change of lily—Lbr-miR156a and its target genesLbrSPL3andLbrSPL16. Research has found that variable temperature treatment can lead toLbr-miR156a downregulates expression,Lbr-miR156a andLbrSPL3LbrSPL16Exhibiting complementary expression patterns。Utilizing subcellular localization and transcription activation bet365 online casinoassays, LbrSPL3 and LbrSPL16 are localized in the nucleus,and has transcriptional activation activity。Discovered by in situ hybridizationLbrSPL3andLbrSPL16Expressed abundantly in the buds of lily bulbs,And its expression abundance was increased after temperature change。And further confirmed by transgenic functional verificationLbr-miR156a and its target genesLbrSPL3LbrSPL16Has the function of regulating phase transition。This research provides new ideas for revealing the phase change mechanism of lily,And provides important scientific basis for the genetic improvement of shortening the juvenile period of lily bulbs。

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Differential expression analysis of known miRNAs and their target genes

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Lbr-miR156a,LbrSPL3ANDLbrSPL16Leaf phenotype of transgenic plants

It is reported,Ornamental Plant Research is an open access journal,Committed to disseminating the latest research progress in the field of ornamental plants,Focus on publishing original research articles in this field、Overview、Comments、Opinion and application papers。The editor-in-chief of the journal is Professor Chen Jianjun of the School of Food and Agricultural Sciences at the University of Florida。

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