Recently，Our school’s Adzuki Bean research team teamed up with BGI，Work closely with the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and Murdoch University, Australia，Utilizing second- and third-generation sequencing technology，Combined Hi-C chromosome-assisted assembly，Successfully completed the genome sequencing and assembly of Adzuki Bean Jingnong 6；simultaneously，Resequencing 322 adzuki bean germplasm resources，Identification of 14 agronomic trait phenotypes，And perform genome-wide association analysis，Get multiple candidate genes from it。Chu Liwei, a graduate of our school、Professor Yang Kai、Associate Professor Zhao Bo，BGI Chen Chunhai is the co-first author of the paper。Professor Wan Ping of our school、Associate Researcher Jian Jianbo of BGI、Researcher Tian Zhixi from the Institute of Genetics and Development, Chinese Academy of Sciences、Professor Rajeev K Varshney of Murdoch University, Australia is the co-corresponding author of the paper。Researcher Qijian Song at the Beltsville Agricultural Research Center of the United States Department of Agriculture bet365 online betting appand Eva C of Swansea University in the United Kingdom. Professor Sonnenschein provided important guidance and help for this research。This research has been approved by the National Key Research and Development Program、National Natural Science Foundation of China、Funded by key projects of Beijing Municipal Education Commission and Beijing Agricultural College and other projects，Academician Gai Junyi and Professor Yang Shouping of Nanjing Agricultural University provided material planting sites for this research。
Adzuki bean (Vigna angularis) originated in China，Is an ancient functional small grain and traditional export crop。The earliest adzuki bean food was discovered at the Kuahuqiao archaeological site in Zhejiang more than 7,000 years ago。Adzuki beans are rich in high-quality protein、Folic acid、Iron、Zinc、Bioactive substances such as adzuki bean saponins and flavonoids，In China、Japan、South Korea、India、Australia、Brazil、Planted in more than 30 countries including Canada。But the origin of adzuki beans is controversial，The domestication mechanism is also unclear，Poor continuity of reference genome。By high-quality genome sequencing、Complete genome assembly and genome-wide association analysis，It can provide important significance for the research on the domestication mechanism of adzuki bean and the improvement of adzuki bean breeding，It also contributes to the domestication and breeding research of other legumes。
This study obtained a high-quality genome at the chromosome level through genome updating，Contig N50 about 16Mb，Serial mount rate reaches 97.bet365 online sports betting app8%， Annotation obtained 32738 protein coding genes。
Figure 1 Genome assembly of Jingnong 6
Researchers use comparative genomics to study the evolution of phylogenetic gene families、Get a comprehensive phylogenetic tree，Unique and shared gene family between adzuki bean and eleven other species identified。Revealing the breakage and fusion of chromosomes 3 and 5 in Adzuki Bean，Discovered that adzuki bean chromosome 5 exists independently in the genus Cowpea，And has an important interval of independent domestication，Probably distinguishes it from species such as mung beans and cowpeas in the genus Vigna。
Figure 2 Comparative genomic analysis of adzuki bean, mung bean and cowpea
The research team studied 322 wild adzuki beans from a wide range of sources、Semi-wild adzuki beans、Whole-genome resequencing analysis of cultivated adzuki bean local varieties and developed varieties，Revealing that China’s wild adzuki beans have high genetic diversity and are the most primitive type，It is speculated that the middle and lower reaches of the Yangtze River in China should be the origin and domestication center of adzuki beans。Constructed a comprehensive adzuki bean genome variation map，Revealing the evolution and domestication patterns of adzuki beans。
Figure 3 Population structure analysis of 322 wild adzuki beans and cultivated adzuki beans
Discovered through research，Adzuki bean chromosome 5 plays an important role in domestication，Candidate gene VaCycA3；1 A single SNP variation is closely related to the pod cracking trait，Candidate gene VaHB15G/T single base SNP variation is related to maturity stage。
Figure 4 Genome-wide association analysis and selection elimination of domestication traits
bet365 games best slotsVaANR1 and VaBm, important candidate genes for adzuki bean seed coat color, were identified on chromosomes 1 and 8 respectively，Haplotype analysis, omics and genetic analysis found that a SNP variation in the coding sequence of the VaANR1 gene may be an important factor in the domestication of red bean coat color。The candidate gene VaBm has 3 haplotypes，The CC haplotype shows no black spots on the seed coat，Wild adzuki bean seed coats all have black spots。
Figure 5 Whole genome analysis of adzuki bean seed coat color
Expressions from teachers and students of the research team，Will fully implement the spirit of the school’s Fourth Party Congress，Implement the deployment requirements of the "Work Implementation Year" in detail，Focus on the "Action Plan for Technological Innovation to Promote High-Quality Development"，Continuously improve scientific research and innovation capabilities，Continuously produce high-level innovative results，Making positive contributions to the construction of a first-class domestic and internationally renowned high-level application-oriented university with urban agriculture and forestry characteristics。
